Season Two was released in June of 2014 on the Syfy Channel (US Viewers). Season Two was released along with a series of mini-episodes that were released prior to Season Two's initial release. They also released a Teaser Trailer and an Official Trailer with it. Season 2 was renewed for a 13-episode order.
- Defiance Minisodes: The Lost Ones
- The Opposite of Hallelujah Season 2, Episode 1
- In My Secret Life Season 2, Episode 2
- The Cord and the Ax Season 2, Episode 3
- Beasts of Burden Season 2, Episode 4
- Put the Damage On Season 2, Episode 5
- This Woman's Work Season 2, Episode 6
- If You Could See Her Through My Eyes Season 2, Episode 7
- Slouching Towards Bethlehem Season 2, Episode 8
- Painted From Memory Season 2, Episode 9
- Bottom of the World Season 2, Episode 10
- Doll Parts Season 2, Episode 11
- All Things Must Pass Season 2, Episode 12
- I Almost Prayed Season 2, Episode 13